|You should write the words as your own words , if you want use figures make sure you should draw it by your hands , make sure to answer every specific thing|
*word limit: 150 words for all Section A (+10% limit)*
State how many times (if any) that each restriction enzyme cuts pSIB2003. Draw apossible resulting restriction map of pSIB2003 (i.e. mark the relative location of the restriction sites on the plasmid), determined from the gel. (5 marks)
What sort of enzyme could be used to fill in the cut fragment’s cohesive ends to produce a flush/blunt end?(2 marks)
Section B (answer ALL questions, each question carries 5 marks)
*word limit: 150 words each question (+10% limit)*
5)Outline the major steps involved in the maintenance of accuracy of DNA replication both during and after DNA synthesis.
6) Describe two ways of making expression of a lac operon constitutive.
Section C(question carries 20 marks)
*word limit: 500 words (+10% limit)*
7) Give an account of chromatin structure, with particular emphasis on how chromatin is modulated to switch genes on and off.
4P Double spaced
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